pcmv ha mammalian expression vector (TaKaRa)
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TaKaRa
pcmv ha mammalian expression vector
Pcmv Ha Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+expression+vector/pCMV-HA+Vector+Set/bio_rxiv__2025__08__02__668297-212-1-15
Average 96 stars, based on 770 article reviews
Pcmv Ha Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcmv+ha+expression+vector/pCMV-HA+Vector+Set/bio_rxiv__2025__08__02__668297-212-1-15
Average 96 stars, based on 770 article reviews
pcmv ha mammalian expression vector - by Bioz Stars,
2026-09
96/100 stars
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Expressing:Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: MEF2B instructs germinal center development and acts as an oncogene in B cell lymphomagenesis Article Snippet: The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into pCMV-HA expression vector (Clontech). .. The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into Article Title: Characterization of clinical envelopes with lack of sensitivity to the HIV-1 inhibitors temsavir and ibalizumab. Article Snippet: .. Products from the secondary PCR were cloned into the Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases. Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics. Article Snippet: Transmembrane protein 168 (TMEM168) was found to be localized on the nuclear membrane.. A heterozygous mutation (c.1616G>A, p. R539Q) in TMEM168 was identified in patients with Brugada syndrome.. This mutation reduced expression of cardiomyocyte sodium channel Nav1.5 via Nedd4-2 E3 ubiquitin ligase-induced ubiquitination and degradation. Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity Article Snippet: .. The envelope genes were cloned into the Article Title: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3 Article Snippet: The plasmid expressing Nedd4l was obtained from Origene. .. TRAF3 and TBK1 were amplified from mRNA of Hela cells and RAW264.7, respectively, by reverse transcription-PCR and the cDNA expressing full-length TRAF3 or TBK1 were cloned into pcDNA3.1-Flag, pCMV-Myc, or Plasmid Preparation:Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: MEF2B instructs germinal center development and acts as an oncogene in B cell lymphomagenesis Article Snippet: The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into pCMV-HA expression vector (Clontech). .. The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into Article Title: Characterization of clinical envelopes with lack of sensitivity to the HIV-1 inhibitors temsavir and ibalizumab. Article Snippet: .. Products from the secondary PCR were cloned into the Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases. Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics. Article Snippet: Transmembrane protein 168 (TMEM168) was found to be localized on the nuclear membrane.. A heterozygous mutation (c.1616G>A, p. R539Q) in TMEM168 was identified in patients with Brugada syndrome.. This mutation reduced expression of cardiomyocyte sodium channel Nav1.5 via Nedd4-2 E3 ubiquitin ligase-induced ubiquitination and degradation. Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity Article Snippet: .. The envelope genes were cloned into the Article Title: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3 Article Snippet: The plasmid expressing Nedd4l was obtained from Origene. .. TRAF3 and TBK1 were amplified from mRNA of Hela cells and RAW264.7, respectively, by reverse transcription-PCR and the cDNA expressing full-length TRAF3 or TBK1 were cloned into pcDNA3.1-Flag, pCMV-Myc, or Clone Assay:Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: Characterization of clinical envelopes with lack of sensitivity to the HIV-1 inhibitors temsavir and ibalizumab. Article Snippet: .. Products from the secondary PCR were cloned into the Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases. Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: Discovery and Characterization of a Novel CD4-Binding Adnectin with Potent Anti-HIV Activity Article Snippet: .. The envelope genes were cloned into the Article Title: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3 Article Snippet: The plasmid expressing Nedd4l was obtained from Origene. .. TRAF3 and TBK1 were amplified from mRNA of Hela cells and RAW264.7, respectively, by reverse transcription-PCR and the cDNA expressing full-length TRAF3 or TBK1 were cloned into pcDNA3.1-Flag, pCMV-Myc, or Transfection:Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Article Title: Quantitative proteome analysis of LAP1-deficient human fibroblasts: A pilot approach for predicting the signaling pathways deregulated in LAP1-associated diseases. Article Snippet: Regarding the secondary antibodies, horseradish peroxidase (HRP)-linked horse anti-mouse immunoglobulin G (IgG; Cell Signaling Technology (7076); 0.015 μg/mL) and HRP-linked goat anti-rabbit IgG (Cell Signaling Technology (7074); 0.007 μg/mL) were used for immunoblotting, whereas Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen (A-11008); 6.67 μg/mL) and Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen (A-11005); 6.67 μg/mL) were utilized for immunocytochemistry. .. Human full-length LAP1B complementary DNA (cDNA) cloned into the pCMV-Myc expression vector (Clontech, Takara Bio USA, San Jose, California, USA) and human full-length LAP1C cDNA cloned into the Sequencing:Article Title: MEF2B instructs germinal center development and acts as an oncogene in B cell lymphomagenesis Article Snippet: The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into pCMV-HA expression vector (Clontech). .. The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into Amplification:Article Title: MEF2B instructs germinal center development and acts as an oncogene in B cell lymphomagenesis Article Snippet: The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into pCMV-HA expression vector (Clontech). .. The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into Article Title: Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics. Article Snippet: Transmembrane protein 168 (TMEM168) was found to be localized on the nuclear membrane.. A heterozygous mutation (c.1616G>A, p. R539Q) in TMEM168 was identified in patients with Brugada syndrome.. This mutation reduced expression of cardiomyocyte sodium channel Nav1.5 via Nedd4-2 E3 ubiquitin ligase-induced ubiquitination and degradation. Article Title: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3 Article Snippet: The plasmid expressing Nedd4l was obtained from Origene. .. TRAF3 and TBK1 were amplified from mRNA of Hela cells and RAW264.7, respectively, by reverse transcription-PCR and the cDNA expressing full-length TRAF3 or TBK1 were cloned into pcDNA3.1-Flag, pCMV-Myc, or Polymerase Chain Reaction:Article Title: MEF2B instructs germinal center development and acts as an oncogene in B cell lymphomagenesis Article Snippet: The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into pCMV-HA expression vector (Clontech). .. The HIRA (GeneBank accession number NM_003325.3) and ASF1a (GeneBank accession number NM_014034.2) coding sequence were amplified by PCR from cDNA of human GC B cells and subcloned into Article Title: Characterization of clinical envelopes with lack of sensitivity to the HIV-1 inhibitors temsavir and ibalizumab. Article Snippet: .. Products from the secondary PCR were cloned into the Article Title: Transmembrane protein 168 mutation reduces cardiomyocyte cell surface expression of Nav1.5 through αB-crystallin intracellular dynamics. Article Snippet: Transmembrane protein 168 (TMEM168) was found to be localized on the nuclear membrane.. A heterozygous mutation (c.1616G>A, p. R539Q) in TMEM168 was identified in patients with Brugada syndrome.. This mutation reduced expression of cardiomyocyte sodium channel Nav1.5 via Nedd4-2 E3 ubiquitin ligase-induced ubiquitination and degradation. Construct:Article Title: E3 ligase Nedd4l promotes antiviral innate immunity by catalyzing K29-linked cysteine ubiquitination of TRAF3 Article Snippet: The plasmid expressing Nedd4l was obtained from Origene. .. TRAF3 and TBK1 were amplified from mRNA of Hela cells and RAW264.7, respectively, by reverse transcription-PCR and the cDNA expressing full-length TRAF3 or TBK1 were cloned into pcDNA3.1-Flag, pCMV-Myc, or |